Real Results: Success Stories from Shuimu’s CryoEM Projects – Unlock High-Resolution Structures with Our Cryo-EM Services

Discover the power of Cryo-Electron Microscopy (Cryo-EM) with Shuimu BioSciences and explore real-world success stories that demonstrate our expertise and commitment to delivering high-resolution structural insights. At Shuimu BioSciences, we offer a comprehensive suite of Cryo-EM services, including One-Stop SPA Solutions, Cryo Characterization, and MicroED Solutions, all designed to accelerate your research and development. Our state-of-the-art facilities, coupled with a team of world-class experts, ensure the highest quality data and structural models.

Ready to elevate your research? Visit https://shuimubio.com/ to learn more about our Cryo-EM services and contact our marketing team for detailed inquiries and project consultations.

Unveiling Macromolecular Structures with One-Stop SPA Solutions

Single Particle Analysis (SPA) is a revolutionary Cryo-EM technique for determining high-resolution 3D structures of biological macromolecules like proteins and viruses. Shuimu BioSciences provides end-to-end SPA solutions, from sample preparation and optimization to data acquisition, processing, and model refinement.

Success Story: Our platform has successfully completed over 300 single-particle projects, showcasing our comprehensive ability to handle diverse structures. We have elucidated protein structures as small as 51 kDa, highlighting our expertise in handling different molecular sizes. This demonstrates our capability to overcome challenges associated with small protein molecular weight, low concentration, high background noise, air-water interface disruption, and preferential orientation using advanced techniques like GraFuture, GO & RGO grids.

The advantages of choosing Shuimu for Cryo-EM SPA include:

• Preservation of native states: Cryo-EM captures samples close to their natural conformation.
• Diverse conformations: We can analyze samples exhibiting conformational heterogeneity.
• Minimal sample volume: SPA requires only small amounts of purified material.
• Heterologous complexes: Determination of structures of complex biomolecular assemblies.
• Cutting-edge equipment: Our Cryo-EM center is equipped with 12 x 300 kV instruments in Beijing and 6 x 300 kV instruments in Hangzhou, including G3i, G4, and Totem microscopes.
• Elite scientist team: Our team comprises PhD-level experts specializing in structural biology, protein science, and computational biology.
• Extensive experience: We have completed over 400 cryo-EM projects, resolving more than 150 structures with a best resolution of 1.8 Å.
• AI-driven platform: Our independently developed SMART software suite streamlines cryo-EM data analysis.

Interested in achieving similar results for your biomacromolecule of interest? Visit https://shuimubio.com/ to explore our SPA services and contact our marketing team to discuss your project feasibility.

Characterizing Nanoparticles with Cryo-EM

Cryo-characterization at Shuimu BioSciences utilizes ultra-low temperature technology to preserve the natural state of nanoparticles like AAV, liposomes, and LNPs for high-resolution structural observation and analysis. Our self-developed NanoSMART AI cryo-EM system automatically identifies nanoparticle features, providing detailed reports on size distribution, roundness, and integrity with one-click operation.

Success Story: Shuimu focuses on the efficient and precise characterization of LNPs, liposomes, AAV, and other viral vectors. Our NanoSMART system ensures clear and detailed reports, meeting diverse customer needs in vaccine development and drug delivery. For example, NanoSMART demonstrates excellent performance in identifying ultra-small targets in VLP samples, achieving a recognition rate close to 100%.

Our Cryo-characterization services offer:

• Detailed analysis of nanoparticle morphology and integrity.
• Accurate measurement of aggregation levels.
• Identification of different liposome and VLP types.
• Statistical dimension display and comprehensive data analysis.

Do you need precise characterization of your nanoparticles? Visit https://shuimubio.com/ to learn more about our Cryo-characterization services and contact our marketing team for sample submission details.

Solving Small Molecule and Peptide Structures with MicroED

Micro-electron diffraction (MicroED) is a cutting-edge technique for determining high-resolution structures from microcrystals and nanocrystals, particularly valuable for small molecule drugs, peptides, and protein crystals. Shuimu BioSciences provides accurate structural insights using MicroED technology, complemented by our proprietary eTasED software that seamlessly integrates with conventional cryo-EM systems.

Success Story: We have successfully delivered over 80% of MicroED projects, achieving resolutions of 0.6~1.0 Å. Our MicroED solutions have been used to resolve the structures of not only small molecules like Acetaminophen but also peptides and proteins like Proteinase K and FUS LC RAC1.

Our MicroED services include:

• Free evaluation services covering feasibility analysis and risk warnings.
• High-resolution structure determination for challenging small molecule samples, peptides, and protein crystals.
• Seamless integration with cryo-EM workflows using eTasED software.

Facing challenges in determining the structure of your small molecules or peptides? Visit https://shuimubio.com/ to explore our MicroED solutions and contact our marketing team for a free evaluation of your project.

Comprehensive Support for Your Structural Biology Research

Beyond these core Cryo-EM techniques, Shuimu BioSciences offers a complete suite of services to support your structural biology research, including:

• Protein Preparation and Analysis Services: From gene cloning to protein expression in various systems (E. coli, mammalian, insect, cell-free) and purification using diverse chromatography techniques. We also provide comprehensive protein characterization assays like SPR, BLI, and ELISA.
• Antibody Discovery: End-to-end services from antigen preparation to antibody generation, expression, purification, and validation.
• One-Stop Crystallographic Analysis: Covering protein expression, purification, crystallization, data collection, and final structure analysis using X-ray crystallography.
• Instrument Access: 24/7 access to our state-of-the-art 300 kV Cryo-EM platforms in Beijing and Hangzhou.

Shuimu BioSciences is committed to providing high-quality Cryo-EM services and supporting your research goals with our experienced team and advanced technologies. Our dedication to pushing the boundaries of structural biology is evident in our numerous successful projects and publications in top international journals.

Ready to experience the Shuimu difference? Visit https://shuimubio.com/ today to explore our full range of services and contact our marketing team to discuss how we can help you achieve your structural biology objectives. We look forward to partnering with you on your next groundbreaking discovery.